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Abstract: Eukaryotic signal transduction involves the assembly of transient protein-protein complexes mediated by modular interaction domains. Specific Pro-rich sequences with the consensus core motif PxxP adopt the PPII helix conformation upon binding to SH3 domains. For short Pro-rich peptides, little or no ordered secondary structure is usually observed before binding interactions. The association of a Pro-rich peptide with the SH3 domain involves unfavorable binding entropy due to the loss of rotational freedom on forming the PPII helix. With the aim of stabilizing the PPII helix conformation in the Pro-rich HPKI decapeptide PPPLPPKPKF (P2), a series of P2 analogues was prepared, in which specific Pro positions were alternatively occupied by 4(S)- or 4(R)-4-fluoro-L-proline. The interactions of these peptides with the SH3 domain of the HPK1-binding partner HS1 were quantitatively analyzed by the NILIA-CD approach. A CD thermal analysis of the P2 analogues was performed to assess their propensity to adopt the PPII helix conformation. Contrary to our expectations, the K-d values of the analogues were lower than that of the parent peptide P2. These results clearly show that the induction of a stable PPII helix conformation in short Pro-rich peptides is not sufficient to increase their affinity toward the SH3 domain and that the effect of 4-fluoroproline strongly depends on the position of this residue in the sequence and the chirality of the substituent in the pyrrolidine ring. Copyright (c) 2006 European Peptide Society and John Wiley & Sons, Ltd.

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Abstract: The search for effective drug delivery systems is one of the major challenges in drug formulation especially for biopharmaceuticals Such as proteins, and peptide-based drugs and vaccines. A procedure has been developed whereby human serum albumin (HSA) call be Used as a delivery vehicle for these biomolecules using its role as main ratty acid carrier. Using essentially fatty acid free HSA (HSAff) it is possible to form stable complexes with lipidic chain compounds (lipo-compounds). Two lipo-compounds have been used to develop this system, a novel antimicrobial lipopeptide and gamma-amino-n-butyric acid, GABA. conjugated with an alkyl chain, lipo-GABA, in both cases C8 and C14 alkyl chain lengths were evaluated. The HAS-lipo compound complex had a mutual stabilizing effect on both the HSA and the lipo-compound. The protease enzyme study showed that the alkyl chains of these lipo-compounds bound to HSAff confer a similar if not greater biostability than caprylic acid shown by CD and importantly, the bound lipopeptide was stabilized by the HSA shown by mass spectrometry. Heat stability studies at 60 degrees C over 10 h also confirmed that the lipo-HSA complexes confer stability and provide a method of preparing sterile formulation for therapeutic use. No further increased in stability of the lipo-compounds when HSA containing fatty acid (HSAfa) Was used. With the antimicrobial lipopeptide, there was enhanced activity with HSAff formulation suggesting increased biostability and bioavailability of compounds. These finding allowed us to develop a simple and effective way of delivering lipo-compounds using fatty acid free HSA as the carrier.

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Abstract: Bacteria are abundant in many natural and engineered environments where they are thought to exert important controls on the cycling, mobility, bioavailability, and toxicity of metal contaminants. In order to probe their role in moderating the behavior of lanthanides, pH-dependent adsorption edges of 13 individual lanthanides and yttrium to the Gram-negative bacterium Pantoea agglomerans were used to generate discrete site surface complexation constants. The calculated surface complexation constants were compared with stability constants estimated using linear free energy relationships based on a number of hydroxyl-containing ligands. The experimental data suggests that lanthanide adsorption edges below pH 6.5 are consistent with adsorption to phosphate groups for the light and some of the middle lanthanides (La to Gd), whereas some of the middle and heavy lanthanides appear to favor carboxyl co-ordination (Tb to Yb), although exceptions occur in each grouping. The experimentally derived surface complexation constants for carboxyl coordination were of similar magnitude to stability constants estimated from linear free energy correlations using fulvic acid stability constants. The implication is that the adsorption of lanthanides to bacterial surfaces could be modeled reasonably well using lanthanide stability constants for natural organic matter, except perhaps at low pH where phosphate binding dominates.